Rat PECAM1/CD31(Platelet/Endothelial Cell Adhesion Molecule 1) EIA Kit

• Catalog number
  E-EL-R0761-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  Platelet endothelial cell adhesion molecule,PECAM-1,cluster of differentiation 31,CD31,CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat PECAM1/CD31. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PECAM1/CD31 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PECAM1/CD31, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PECAM1/CD31. You can calculate the concentration of Rat PECAM1/CD31 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  18.75pg/mL
• Detection Range
  31.25~2000pg/mL
• Target Name
  PECAM1/CD31
• UNIProt ID
  Q3SWT0
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat PECAM1/CD31(Platelet/Endothelial Cell Adhesion Molecule 1) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the PECAM1/CD31(Platelet/Endothelial Adhesion Molecule 1) EIA Kit. For cells, cell lines and tissues in culture till half confluency. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  PECAM1/CD31   Platelet/Endothelial   Adhesion   Molecule   Kit  
• Gene symbol
  PECAM1
• Short name
  PECAM1/CD31(Platelet/Endothelial Adhesion Molecule 1) EIA Kit
• Technique
  EIA, eia
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat PECAM1/CD31(Platelet/Endothelial cellular Adhesion Molecule 1) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

Gene info

• Identity
  HGNC:8823
• Gene
  PECAM1
• Long gene name
  platelet and endothelial cell adhesion molecule 1
• Synonyms gene name
  • platelet/endothelial cell adhesion molecule 1
• Synonyms
  • CD31
• Synonyms name
  • CD31 antigen
• GenBank acession
  • L34631
  • M37780
• Locus
  17q23.3
• Discovery year
  1995-11-29
• Entrez gene record
  5175
• Pubmed identfication
  • 8661055
  • 1690453
• RefSeq identity
  • NM_000442
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
  • Ig-like cell adhesion molecule family
• VEGA ID
  OTTHUMG00000175829

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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