Rat GzmB(Granzyme B) EIA Kit
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Catalog number
E-EL-R0456-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
GZM-B, HLP, CTLA1, CCPI, CGL1, CSP-B, CSPB, CTSGL1, SECT, Granzyme 2, Cathepsin G-like 1,T-cell serine protease 1-3E
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat GzmB. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GzmB. You can calculate the concentration of Rat GzmB in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
9.38pg/mL
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Detection Range
15.63~1000pg/mL
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Target Name
GzmB
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UNIProt ID
P18291
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat GzmB(Granzyme B) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
GzmB(Granzyme B) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)(Granzyme B) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), CCPI and CGL-1 and CGL1 and CSP-B and CSPB and CTLA1 and CTSGL1 and HLP and SECT, GZMB and IDBG-4054 and ENSG00000100453 and 3002, serine-type peptidase activity, nuclei, BT.19365 and IDBG-632083 and ENSBTAG00000010057 and 508646
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MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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