Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit

• Catalog number
  E-CL-R0008-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  GMCSF, CSF2
• Test principle
  This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GM-CSF . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GM-CSF , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GM-CSF . You can calculate the concentration of Rat GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.
• Sensitivity
  4.69pg/mL
• Detection Range
  7.81~500pg/mL
• Target Name
  GM-CSF
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Gene
  Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  GM-CSF   Granulocyte-Macrophage   Colony   Stimulating   Factor   CLIA   Kit  
• Gene symbol
  CSF2, PPP1R3A, CSF1, CSF3, IL3
• Short name
  GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  granulocyte

Gene info

• Identity
  HGNC:2434
• Gene
  CSF2
• Long gene name
  colony stimulating factor 2
• Synonyms gene name
  • colony stimulating factor 2 (granulocyte-macrophage)
• Synonyms
  • GM-CSF
  • GMCSF
• Synonyms name
  • sargramostim
  • molgramostim
  • granulocyte-macrophage colony stimulating factor
• GenBank acession
  • M11734
• Locus
  5q31.1
• Discovery year
  2001-06-22
• Entrez gene record
  1437
• Pubmed identfication
  • 3898082
  • 2999978
• RefSeq identity
  • NM_000758
• VEGA ID
  OTTHUMG00000059637

Gene info

• Identity
  HGNC:9291
• Gene
  PPP1R3A
• Long gene name
  protein phosphatase 1 regulatory subunit 3A
• Synonyms gene
  • PPP1R3
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 3A (glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle)
  • protein phosphatase 1, regulatory (inhibitor) subunit 3A
  • protein phosphatase 1, regulatory subunit 3A
• Synonyms
  • GM
• Synonyms name
  • glycogen-associated regulatory subunit of protein phosphatase-1
  • protein phosphatase 1 regulatory subunit GM
• GenBank acession
  • AF024578
• Locus
  7q31.1
• Discovery year
  1993-01-22
• Entrez gene record
  5506
• Pubmed identfication
  • 7926294
• RefSeq identity
  • NM_002711
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000156944

Gene info

• Identity
  HGNC:2432
• Gene
  CSF1
• Long gene name
  colony stimulating factor 1
• Synonyms gene name
  • colony stimulating factor 1 (macrophage)
• Synonyms
  • M-CSF
  • MCSF
  • MGC31930
• Synonyms name
  • macrophage colony stimulating factor 1
• GenBank acession
  • BC021117
• Locus
  1p13.3
• Discovery year
  1986-01-01
• Entrez gene record
  1435
• Pubmed identfication
  • 1540160
• RefSeq identity
  • NM_000757
• VEGA ID
  OTTHUMG00000011646

Gene info

• Identity
  HGNC:2438
• Gene
  CSF3
• Long gene name
  colony stimulating factor 3
• Synonyms gene
  • GCSF
  • G-CSF
  • C17orf33
• Synonyms gene name
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
• Synonyms
  • MGC45931
• Synonyms name
  • granulocyte colony stimulating factor
  • pluripoietin
  • filgrastim
  • lenograstim
• Locus
  17q21.1
• Discovery year
  2001-06-22
• Entrez gene record
  1440
• Pubmed identfication
  • 3499671
  • 3501046
• RefSeq identity
  • NM_172220
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000133247

Gene info

• Identity
  HGNC:6011
• Gene
  IL3
• Long gene name
  interleukin 3
• Synonyms gene name
  • interleukin 3 (colony-stimulating factor, multiple)
• Synonyms
  • IL-3
  • MULTI-CSF
  • MCGF
  • MGC79398
  • MGC79399
• Synonyms name
  • multilineage-colony-stimulating factor
  • hematopoietic growth factor
  • P-cell stimulating factor
  • mast-cell growth factor
  • colony-stimulating factor, multiple
• GenBank acession
  • M14743
• Locus
  5q31.1
• Discovery year
  2001-06-22
• Entrez gene record
  3562
• Pubmed identfication
  • 3489530
• RefSeq identity
  • NM_000588
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000059640

MeSH Data

• Name
  Colony-Forming Units Assay
• Concept
  Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
• Tree numbers
  • E01.370.225.500.383
  • E05.200.500.383
  • E05.242.383
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products