Rat F1+2(Prothrombin Fragment 1+2) EIA Kit
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Catalog numberE-EL-R1206-96T
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Price:
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Size96Test
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Detection TypeColormetric
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Target SpeciesRat
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Target SynonymF1+2
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat F1+2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F1+2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F1+2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat F1+2. You can calculate the concentration of Rat F1+2 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity28.13pg/mL
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Detection Range46.88~3000pg/mL
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Target NameF1+2
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UNIProt IDNA
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameRat F1+2(Prothrombin Fragment 1+2) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Short nameF1+2(Prothrombin 1+2) EIA Kit
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TechniqueEIA, eia
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HostRat
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IsotypeAntibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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SpeciesRat, Rats
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Alternative nameRat F1and2(Prothrombin Fragment 1and2) EIA reagent
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Alternative techniquekits, elisas
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data