Rat Cathepsin B / CTSB Protein (His Tag) 50µg
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Catalog number
PKSR030155-50µg
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Price
Please ask
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Size
50µg
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Synonym
Cathepsin B;CTSB
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Activity
NA
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Sequence
Met1-Phe339
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Fusion tag
C-His
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Accession
NP_072119.2
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ExpressedHost
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 95 % as determined by SDS-PAGE.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
37.1 kDa
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AP Mol Mass
37.1 kDa
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Formulation
Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5
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Background
Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
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Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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Conjugation
histidine
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
CTSB
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Short name
Cathepsin B / CTSB Protein (His Tag) 50µg
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Technique
Tags
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Host
Rat
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Label
His
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Species
Rat, Rats
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Alternative name
Rat Cathepsin B / cathepsin B Protein (histidine detection labelled) 50µg
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Alternative to gene target
cathepsin B, APPS and CPSB, CTSB and IDBG-7654 and ENSG00000164733 and 1508, proteoglycan binding, nuclei, Ctsb and IDBG-172314 and ENSMUSG00000021939 and 13030, CTSB and IDBG-629395 and ENSBTAG00000012442 and 281105
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Gene info
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Identity
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Gene
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Long gene name
cathepsin B
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Product images
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