Rat C9(Complement Component 9) EIA Kit
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Catalog number
E-EL-R0252-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat C9. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C9. You can calculate the concentration of Rat C9 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
5.63ng/mL
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Detection Range
9.38~600ng/mL
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Target Name
C9
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UNIProt ID
Q62930
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat C9(Complement Component 9) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Short name
C9(Complement Component 9) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat complement component 9(Complement Component 9) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
complement component 9, ARMD15 and C9D, C9 and IDBG-17892 and ENSG00000113600 and 735, protein binding, Extracellular, C9 and IDBG-128081 and ENSMUSG00000022149 and 12279, C9 and IDBG-629942 and ENSBTAG00000016149 and 526766
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MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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