Porcine GnRH(Gonadotropin Releasing Hormone) EIA Kit

• Catalog number
  E-EL-P1291-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Porcine
• Target Synonym
  GNRH1, GRH, HH12, LHRH, LNRH, Luliberin
• Test principle
  This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Porcine GnRH. During the reaction, Porcine GnRH in the sample or standard competes with a fixed amount of Porcine GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine GnRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sensitivity
  37.5pg/mL
• Detection Range
  62.5~4000pg/mL
• Target Name
  GnRH
• UNIProt ID
  P49921
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Porcine GnRH(Gonadotropin Releasing Hormone) EIA Kit
• Type
  Competitive
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

• Gene target
  GnRH   Gonadotropin   Releasing   Hormone   Kit  
• Short name
  Porcine GnRH(Gonadotropin Releasing Hormone) EIA Kit
• Technique
  EIA, eia
• Species
  Porcine
• Alternative name
  swine GnRH(Gonadotropin Releasing Hormone) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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