P19 [P-19] Cell Line

• Catalog number
  EP-CL-0179
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  P19 [P-19]
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The line can be cloned at high efficiency in medium containing 0.1mM 2-mercaptoethanol.The cells are pluripotent.The cell can be induced to differentiate into neural and glial like cells in the presence of 500 nM retinoic acid.In the presence of 0.5% to 1.0% dimethylsulfoxide(DMSO) the cells differentiate to form cardiac and skeletal muscle-like elements, but do not form neural or glial like cells.In the presence of both DMSO and retinoic acid, the cells differentiate as in the presence of retinoic acid alone.
• Biosafety
  1
• Medium
  MEMα (PM150421)+7.5% CS+2.5% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  P19   [P-19]   Line  
• Gene symbol
  H3P13, CDKN2D, IL23A, PIRC99, PIRC98, PIRC100, PIRC97, PIRC101
• Short name
  P19 [P-19] Line
• Species
  Mus musculus, Mouse
• Alternative name
  P19 [P-19] cellular Line
• Tissue
  Embryo

Gene info

• Identity
  HGNC:43804
• Gene
  H3P13
• Long gene name
  H3 histone pseudogene 13
• Synonyms
  • p19
• Locus
  3q26.33
• Discovery year
  2019-08-02
• Entrez gene record
  115482685
• Pubmed identfication
  • 21782046
• RefSeq identity
  • NG_066038

Gene info

• Identity
  HGNC:1790
• Gene
  CDKN2D
• Long gene name
  cyclin dependent kinase inhibitor 2D
• Synonyms gene name
  • cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• Synonyms
  • INK4D
  • p19
• Locus
  19p13.2
• Discovery year
  1995-07-06
• Entrez gene record
  1032
• Pubmed identfication
  • 8575754
• RefSeq identity
  • NM_079421
• Classification
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000180583

Gene info

• Identity
  HGNC:15488
• Gene
  IL23A
• Long gene name
  interleukin 23 subunit alpha
• Synonyms gene name
  • interleukin 23, alpha subunit p19
• Synonyms
  • SGRF
  • IL23P19
  • IL-23
  • IL-23A
  • P19
• Synonyms name
  • interleukin-six, G-CSF related factor
• GenBank acession
  • AB030000
• Locus
  12q13.3
• Discovery year
  2001-04-10
• Entrez gene record
  51561
• Pubmed identfication
  • 11114383
• RefSeq identity
  • NM_016584
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000187402

Gene info

• Identity
  HGNC:37603
• Gene
  PIRC99
• Long gene name
  piwi-interacting RNA cluster 99
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313875
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37602
• Gene
  PIRC98
• Long gene name
  piwi-interacting RNA cluster 98
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313874
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37604
• Gene
  PIRC100
• Long gene name
  piwi-interacting RNA cluster 100
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313912
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37601
• Gene
  PIRC97
• Long gene name
  piwi-interacting RNA cluster 97
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313821
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37605
• Gene
  PIRC101
• Long gene name
  piwi-interacting RNA cluster 101
• Locus
  19
• Discovery year
  2009-11-05
• Entrez gene record
  100313888
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

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