NR8383 [AgC11x3A; NR8383.1] Cell Line
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Catalog number
EP-CL-0172
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Price
Please ask
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Size
1×10⁶ cells/vial
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Abbreviation
NR8383 [AgC11x3A; NR8383.1]
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
NA
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Morphology
Macrophage
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Growth_properties
Mixed, Adherent And Suspension
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Doubling_time
NA
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Background
The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.Subsequently the requirement for exogenous growth factors was lost.The cells exhibit characteristics of macrophage cells.Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.NR8383 cells respond to bleomycin by secreting latent transforming growth factor(TGF beta).Stimulation with bleomycin also increases TGF beta mRNA expression.These cells are sensitive to endotoxin.LPS levels of 1 to 10 ng/mL inhibit replication by 50%.LPS inhibition is nontoxic and reversible even after levels up to 0.001mg/mL for extended periods.
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Biosafety
1
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Medium
Ham's F-12K (PM150910)+20% heat-inactivated FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
NA
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Receptor_expression
Fc
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Antigen_expression
NA
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Gene_expression
Transforming Growth Factor Beta(Tgf Beta); Interleukin 1(Il-1); Interleukin 6(Il-6)
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Applications
The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
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