NK-92MI Cell Line

• Catalog number
  EP-CL-0533
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  NK-92MI
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  50 years
• Morphology
  Lymphoblast
• Growth_properties
  Suspension, Multicell Aggregates
• Doubling_time
  NA
• Background
  NK-92 and this derivative cell line NK-92MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. The parental IL-2 dependent cell line is available as NK-92. NK-92MI was shown to contain, express, and synthesize the hIL-2. A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
• Biosafety
  2
• Medium
  MEMα (PM150421)+0.2mM Inositol+0.1mM β-mercaptoethanol (PB180633)+0.02mM Folic Acid+12.5% HS (164215-100)+12.5% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  The cell line is cytotoxic to a wide range of malignant cells. it kills both K562 cells and Daudi cells in chromium release assays.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  NK-92MI   Line  
• Gene symbol
  NCR2, NCR1, B3GAT1
• Short name
  NK-92MI Line
• Species
  Homo sapiens, Human
• Alternative name
  NK-92MI cellular Line
• Tissue
  Peripheral blood, blood

Gene info

• Identity
  HGNC:6732
• Gene
  NCR2
• Long gene name
  natural cytotoxicity triggering receptor 2
• Synonyms gene
  • LY95
• Synonyms gene name
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
• Synonyms
  • NK-p44
  • CD336
• GenBank acession
  • AJ225109
• Locus
  6p21.1
• Discovery year
  1999-04-09
• Entrez gene record
  9436
• Pubmed identfication
  • 10049942
• Classification
  • CD molecules
  • V-set domain containing
• VEGA ID
  OTTHUMG00000014678

Gene info

• Identity
  HGNC:6731
• Gene
  NCR1
• Long gene name
  natural cytotoxicity triggering receptor 1
• Synonyms gene
  • LY94
• Synonyms gene name
  • lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
• Synonyms
  • NK-p46
  • NKP46
  • CD335
• GenBank acession
  • AJ001383
• Locus
  19q13.42
• Discovery year
  1999-04-09
• Entrez gene record
  9437
• Pubmed identfication
  • 9730896
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000183212

Gene info

• Identity
  HGNC:921
• Gene
  B3GAT1
• Long gene name
  beta-1,3-glucuronyltransferase 1
• Synonyms gene
  • CD57
  • LEU7
• Synonyms gene name
  • CD57 antigen
• Synonyms
  • GlcAT-P
  • HNK-1
  • NK-1
• Synonyms name
  • galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
  • glucuronosyltransferase P
• GenBank acession
  • AB029396
• Locus
  11q25
• Discovery year
  2000-01-07
• Entrez gene record
  27087
• RefSeq identity
  • NM_018644
• Classification
  • Beta-1,3-glucuronyltransferases
  • CD molecules
• VEGA ID
  OTTHUMG00000167180

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