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Abbreviation
MS751
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
47 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
MS751 cells have been reported to contain human papilloma virus 18(HPV-18) sequences. More recently, it has been shown that MS751 cells contain a partial HPV-45 genome, and that HPV-45 sequences from the E6/E7 region are expressed as poly(A)＋RNA.
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Biosafety
2
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Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (gradeⅢ).
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
Blood Type AB; Rh＋
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Gene_expression
NA
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
