Mouse PDGF-B(Platelet Derived Growth Factor Subunit B) EIA Kit

• Catalog number
  E-EL-M0924-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Mouse
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGF-B. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PDGF-B. You can calculate the concentration of Mouse PDGF-B in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  18.75 pg/mL
• Detection Range
  31.25~2000pg/mL
• Target Name
  PDGF-B
• UNIProt ID
  P31240
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Mouse PDGF-B(Platelet Derived Growth Factor Subunit B) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  PDGF-B   Platelet   Derived   Growth   Factor   Subunit   Kit  
• Gene symbol
  PDGFB, PDGFA, SNRPB, ORM2, ERAL1, OR1B1, SPIB, EXPH5, HLA-B, RELB
• Short name
  Mouse PDGF-B(Platelet Derived Growth Factor Subunit B) EIA Kit
• Technique
  Mouse, EIA, eia, mouses
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Mouse PDGF-B(Platelet Derived Growth Factor functionnal sequence B) EIA reagent
• Alternative technique
  murine, kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:8800
• Gene
  PDGFB
• Long gene name
  platelet derived growth factor subunit B
• Synonyms gene
  • SIS
• Synonyms gene name
  • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
  • platelet-derived growth factor beta polypeptide
• Synonyms
  • SSV
• Synonyms name
  • oncogene SIS
  • becaplermin
• Locus
  22q13.1
• Discovery year
  2001-06-22
• Entrez gene record
  5155
• Pubmed identfication
  • 2991848
  • 1661670
• RefSeq identity
  • NM_002608
• VEGA ID
  OTTHUMG00000151029

Gene info

• Identity
  HGNC:8799
• Gene
  PDGFA
• Long gene name
  platelet derived growth factor subunit A
• Synonyms gene name
  • platelet-derived growth factor alpha polypeptide
• Synonyms
  • PDGF1
  • PDGF-A
• Synonyms name
  • PDGF A-chain
  • platelet-derived growth factor alpha chain
• Locus
  7p22.3
• Discovery year
  1986-01-01
• Entrez gene record
  5154
• Pubmed identfication
  • 1505216
  • 2536956
• VEGA ID
  OTTHUMG00000151412

Gene info

• Identity
  HGNC:11153
• Gene
  SNRPB
• Long gene name
  small nuclear ribonucleoprotein polypeptides B and B1
• Synonyms gene
  • SNRPB1
• Synonyms
  • COD
  • SmB/SmB'
  • Sm-B/B'
  • snRNP-B
• Locus
  20p13
• Discovery year
  1988-11-28
• Entrez gene record
  6628
• Pubmed identfication
  • 1376292
• Classification
  • Small nucleolar RNA protein coding host genes
  • Sm spliceosomal proteins
• VEGA ID
  OTTHUMG00000031694

Gene info

• Identity
  HGNC:8499
• Gene
  ORM2
• Long gene name
  orosomucoid 2
• Synonyms
  • AGP-B
  • AGP-B'
  • AGP2
• Synonyms name
  • alpha-1-acid glycoprotein, type 2
• Locus
  9q32
• Discovery year
  1991-03-11
• Entrez gene record
  5005
• Pubmed identfication
  • 4711474
  • 2970990
• RefSeq identity
  • NM_000608
• Classification
  • Lipocalins
• VEGA ID
  OTTHUMG00000021014

Gene info

• Identity
  HGNC:3424
• Gene
  ERAL1
• Long gene name
  Era like 12S mitochondrial rRNA chaperone 1
• Synonyms gene name
  • Era (E. coli G-protein homolog)-like 1
  • Era G-protein-like 1 (E. coli)
  • Era-like 12S mitochondrial rRNA chaperone 1
• Synonyms
  • HERA-B
• GenBank acession
  • AF082657
• Locus
  17q11.2
• Discovery year
  1999-11-19
• Entrez gene record
  26284
• Pubmed identfication
  • 10945472
  • 20604745
• VEGA ID
  OTTHUMG00000132675

Gene info

• Identity
  HGNC:8181
• Gene
  OR1B1
• Long gene name
  olfactory receptor family 1 subfamily B member 1
• Synonyms gene name
  • olfactory receptor, family 1, subfamily B, member 1 (gene/pseudogene)
  • olfactory receptor family 1 subfamily B member 1 (gene/pseudogene)
• Synonyms
  • OR9-B
• GenBank acession
  • AC006313
• Locus
  9q33.2
• Discovery year
  1999-12-09
• Entrez gene record
  347169
• RefSeq identity
  • NM_001004450
• Classification
  • Olfactory receptors, family 1
• VEGA ID
  OTTHUMG00000020616

Gene info

• Identity
  HGNC:11242
• Gene
  SPIB
• Long gene name
  Spi-B transcription factor
• Synonyms gene name
  • Spi-B transcription factor (Spi-1/PU.1 related)
• Synonyms
  • SPI-B
• Locus
  19q13.33
• Discovery year
  1994-12-15
• Entrez gene record
  6689
• Pubmed identfication
  • 1406622
• RefSeq identity
  • NM_003121
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000183039

Gene info

• Identity
  HGNC:30578
• Gene
  EXPH5
• Long gene name
  exophilin 5
• Synonyms
  • SLAC2-B
• Synonyms name
  • synaptotagmin-like homologue lacking C2 domains b
• Locus
  11q22.3
• Discovery year
  2005-10-04
• Entrez gene record
  23086
• Pubmed identfication
  • 9734811
  • 11773082
• RefSeq identity
  • NM_015065
• VEGA ID
  OTTHUMG00000166536

Gene info

• Identity
  HGNC:4932
• Gene
  HLA-B
• Long gene name
  major histocompatibility complex, class I, B
• Synonyms gene
  • AS
• Synonyms gene name
  • ankylosing spondylitis
• GenBank acession
  • M15470
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  3106
• Pubmed identfication
  • 3459708
• RefSeq identity
  • NM_005514
• Classification
  • C1-set domain containing
  • Histocompatibility complex
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000031153
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:9956
• Gene
  RELB
• Long gene name
  RELB proto-oncogene, NF-kB subunit
• Synonyms gene name
  • v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
• Synonyms
  • REL-B
• GenBank acession
  • M83221
• Locus
  19q13.32
• Discovery year
  1995-10-02
• Entrez gene record
  5971
• Pubmed identfication
  • 1531086
• Classification
  • IPT domain containing
  • NF-kappa B complex subunits
• VEGA ID
  OTTHUMG00000162116

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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