Mouse PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit

• Catalog number
  E-EL-M1358-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Mouse
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PACAP-27. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PACAP-27. You can calculate the concentration of Mouse PACAP-27 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.19 ng/mL
• Detection Range
  0.313~20ng/mL
• Target Name
  PACAP-27
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Mouse PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  PACAP-27   Pituitary   Adenylate   Cyclase   Activating   Polypeptide   Kit  
• Gene symbol
  ADCYAP1, SNORD115-27, IGKV1D-27, RNVU1-27, IGHV7-27, ERVK-27, ERVW-27, SNORD114-27, SNORD116-27, IGLV3-27
• Short name
  Mouse PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit
• Technique
  Mouse, EIA, eia, mouses
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Mouse PACAP-27(Pituitary Adenylate Cyclase Activating multipeptide 27) EIA reagent
• Alternative technique
  murine, kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:241
• Gene
  ADCYAP1
• Long gene name
  adenylate cyclase activating polypeptide 1
• Synonyms gene name
  • adenylate cyclase activating polypeptide 1 (pituitary)
• Synonyms
  • PACAP
• Synonyms name
  • prepro-PACAP
  • pituitary adenylate cyclase activating polypeptide
• GenBank acession
  • S83513
• Locus
  18p11.32
• Discovery year
  1992-12-02
• Entrez gene record
  116
• Pubmed identfication
  • 1730060
• RefSeq identity
  • NM_001117
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000131479

Gene info

• Identity
  HGNC:33046
• Gene
  SNORD115-27
• Long gene name
  small nucleolar RNA, C/D box 115-27
• Synonyms
  • HBII-52-27
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100036564
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003496
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5751
• Gene
  IGKV1D-27
• Long gene name
  immunoglobulin kappa variable 1D-27 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1D-27
• GenBank acession
  • Z00004
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28898
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151607

Gene info

• Identity
  HGNC:54431
• Gene
  RNVU1-27
• Long gene name
  RNA, variant U1 small nuclear 27
• Locus
  1q21.2
• Discovery year
  2019-07-26
• Classification
  • Variant U1 small nuclear RNAs

Gene info

• Identity
  HGNC:5663
• Gene
  IGHV7-27
• Long gene name
  immunoglobulin heavy variable 7-27 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 7-27
  • immunoglobulin heavy variable 7-27 pseudogene
• GenBank acession
  • M99643
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28383
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152070

Gene info

• Identity
  HGNC:44980
• Gene
  ERVK-27
• Long gene name
  endogenous retrovirus group K member 27
• Synonyms gene name
  • endogenous retrovirus group K, member 27
• GenBank acession
  • JZ164909
• Locus
  11q12.3
• Discovery year
  2014-01-03
• Pubmed identfication
  • 23338945

Gene info

• Identity
  HGNC:49315
• Gene
  ERVW-27
• Long gene name
  endogenous retrovirus group W member 27
• Synonyms gene name
  • endogenous retrovirus group W, member 27
• GenBank acession
  • HG421064
• Locus
  14q32.12
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:33015
• Gene
  SNORD114-27
• Long gene name
  small nucleolar RNA, C/D box 114-27
• Synonyms
  • 14q(II-27)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767608
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003220.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33093
• Gene
  SNORD116-27
• Long gene name
  small nucleolar RNA, C/D box 116-27
• Synonyms
  • HBII-85-27
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033439
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003341
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5910
• Gene
  IGLV3-27
• Long gene name
  immunoglobulin lambda variable 3-27
• GenBank acession
  • D86994
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28791
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151171

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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