Mouse HB-EGF(Heparin-binding Epidermal Growth Factor-like Growth Factor) EIA Kit
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Catalog number
E-EL-M0658-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Mouse
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HB-EGF. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HB-EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HB-EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HB-EGF. You can calculate the concentration of Mouse HB-EGF in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
9.38 pg/mL
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Detection Range
15.625~1000pg/mL
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Target Name
HB-EGF
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UNIProt ID
Q06186
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Mouse HB-EGF(Heparin-binding Epidermal Growth Factor-like Growth Factor) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
EGF, HMHB1, KRT84, HBEGF, KRT85, KRT81, KRT82, KRT83, PEG10
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Short name
Mouse HB-EGF(Heparin-binding Epidermal Growth Factor-like Growth Factor) EIA Kit
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Technique
Mouse, EIA, eia, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Mouse HB-epidermal growth factor(Heparin-binding Epidermal Growth Factor-like Growth Factor) EIA reagent
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Alternative technique
murine, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
epidermal growth factor
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Synonyms gene name
- epidermal growth factor (beta-urogastrone)
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
keratin 84
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Synonyms gene
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Synonyms gene name
- keratin, hair, basic, 4
- keratin 84, type II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
keratin 85
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Synonyms gene
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Synonyms gene name
- keratin, hair, basic, 5
- keratin 85, type II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 81
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Synonyms gene
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Synonyms gene name
- keratin, hair, basic, 1
- keratin 81, type II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 82
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Synonyms gene
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Synonyms gene name
- keratin, hair, basic, 2
- keratin 82, type II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
keratin 83
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Synonyms gene
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Synonyms gene name
- keratin, hair, basic, 3
- keratin 83, type II
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
paternally expressed 10
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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