Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit

  • Catalog number
    E-CL-M0030-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Mouse
  • Target Synonym
    GMCSF, CSF2
  • Test principle
    This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GM-CSF . You can calculate the concentration of Mouse GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.
  • Sensitivity
    4.69pg/mL
  • Detection Range
    7.81~500pg/mL
  • Target Name
    GM-CSF
  • UNIProt ID
    NA
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
  • Type
    Sandwich
  • Technical data
  • Standard Curve RUL
  • Description
    Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Gene
    Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    GM-CSF   Granulocyte-Macrophage   Colony   Stimulating   Factor   CLIA   Kit  
  • Gene symbol
    CSF2, PPP1R3A, CSF1, CSF3, IL3
  • Short name
    Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit
  • Technique
    Mouse, mouses
  • Host
    mouse
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA reagent
  • Alternative technique
    murine, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    granulocyte
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Colony-Forming Units Assay
  • Concept
    Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
  • Tree numbers
    • E01.370.225.500.383
    • E05.200.500.383
    • E05.242.383
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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