Mouse CAD(Caspase Activated Deoxyribonuclease) EIA Kit

  • Catalog number
    E-EL-M0245-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Mouse
  • Target Synonym
    NA
  • Test principle
    This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CAD. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CAD. You can calculate the concentration of Mouse CAD in the samples by comparing the OD of the samples to the standard curve.
  • Sensitivity
    0.94 ng/mL
  • Detection Range
    1.563~100ng/mL
  • Target Name
    CAD
  • UNIProt ID
    O54788 
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Mouse CAD(Caspase Activated Deoxyribonuclease) EIA Kit
  • Type
    Sandwich
  • Description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    CALD1, CAD, B4GALNT2, ACOD1, CDH12P2, DFFB
  • Short name
    Mouse CAD(Caspase Activated Deoxyribonuclease) EIA Kit
  • Technique
    Mouse, EIA, eia, mouses
  • Host
    mouse
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse CAD(caspase Activated Deoxyribonuclease) EIA reagent
  • Alternative technique
    murine, kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
  • Identity
  • Gene
    CAD
  • Long gene name
    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
    790
  • Pubmed identfication
  • Classification
    • Glutamine amidotransferase class 1 domain containing
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
  • Tree numbers
    • E05.478.566.350
    • E05.478.583.400
    • E05.601.470.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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