MDA-MB-468 Cell Line
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Catalog numberEP-CL-0290
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationMDA-MB-468
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age51 years
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundAlthough the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution. EGF receptor is present at 1×10^6 per cell.
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).
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Receptor_expressionEpidermal growth factor(EGF); transforming growth factor alpha(TGF alpha)
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Antigen_expressionBlood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4(patient)
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Gene_expressionNA
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolMB, CD79A, SDCBP, IFIH1, IL24
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Short nameMDA-MB-468 Line
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SpeciesHomo sapiens, Human
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Alternative nameMDA-myoglobin-468 cellular Line
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Alternative to gene targetmyoglobin, myoglobgin and PVALB, MB and IDBG-5491 and ENSG00000198125 and 4151, heme binding, multiple, Mb and IDBG-157509 and ENSMUSG00000018893 and 17189, MB and IDBG-637940 and ENSBTAG00000005333 and 280695
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TissueMammary gland/breast, derived from metastatic site: pleural effusion
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Gene info
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Identity
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Gene
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Long gene namemyoglobin
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD79a molecule
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Synonyms gene
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Synonyms gene name
- CD79A antigen (immunoglobulin-associated alpha)
- CD79a molecule, immunoglobulin-associated alpha
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-07-31
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- V-set domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesyndecan binding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterferon induced with helicase C domain 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-06-25
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Entrez gene record
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RefSeq identity
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Classification
- RNA helicases
- Caspase recruitment domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 24
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID