L Wnt-3A Cell Line

• Catalog number
  EP-CL-0375
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  L Wnt-3A
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  100 days
• Morphology
  Fibroblast
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development. The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium. Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line.
• Biosafety
  1
• Medium
  DMEM (PM150210)+0.4mg/mL G418+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Wnt-3A
• Applications
  This cell line is presently the best source for production of Wnt-3A conditioned medium.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Wnt-3A   Line  
• Gene symbol
  IGHD3OR15-3A, H3-3A-DT, H3-3A, FAM102A, CCL20
• Short name
  L Wnt-3A Line
• Species
  Mus musculus, Mouse
• Alternative name
  L Wnt-3A cellular Line
• Tissue
  Subcutaneous connective tissue, areolar and adipose

Gene info

• Identity
  HGNC:5500
• Gene
  IGHD3OR15-3A
• Long gene name
  immunoglobulin heavy diversity 3/OR15-3A (non-functional)
• Synonyms gene name
  • immunoglobulin heavy diversity 3/OR15-3A
• Synonyms
  • IGHD3/OR15-3A
  • IGHD3OR153A
• GenBank acession
  • X55579
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28331
• Pubmed identfication
  • 2114977
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000184598

Gene info

• Identity
  HGNC:55210
• Gene
  H3-3A-DT
• Long gene name
  H3-3A divergent transcript
• Locus
  1q42.12
• Discovery year
  2020-08-21
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4764
• Gene
  H3-3A
• Long gene name
  H3.3 histone A
• Synonyms gene
  • H3F3
  • H3F3A
• Synonyms gene name
  • H3 histone, family 3A
  • H3 histone family member 3A
• Synonyms
  • H3.3A
• GenBank acession
  • BC029405
• Locus
  1q42.12
• Discovery year
  1989-12-11
• Entrez gene record
  3020
• Pubmed identfication
  • 3031613
• RefSeq identity
  • NM_002107
• Classification
  • H3 histones
• VEGA ID
  OTTHUMG00000037507
• Locus Specific Databases
  • LRG_1410

Gene info

• Identity
  HGNC:31419
• Gene
  FAM102A
• Long gene name
  family with sequence similarity 102 member A
• Synonyms gene
  • C9orf132
• Synonyms gene name
  • chromosome 9 open reading frame 132
  • family with sequence similarity 102, member A
• Synonyms
  • Eeig1
  • bA203J24.7
  • SYM-3A
• Synonyms name
  • sym-3 homolog A (C. elegans)
  • early estrogen-induced gene 1
• Locus
  9q34.11
• Discovery year
  2005-03-22
• Entrez gene record
  399665
• Pubmed identfication
  • 23478294
• Classification
  • NT-C2 domain containing
• VEGA ID
  OTTHUMG00000020720

Gene info

• Identity
  HGNC:10619
• Gene
  CCL20
• Long gene name
  C-C motif chemokine ligand 20
• Synonyms gene
  • SCYA20
• Synonyms gene name
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • chemokine (C-C motif) ligand 20
• Synonyms
  • LARC
  • MIP-3a
  • exodus-1
  • ST38
  • CKb4
• GenBank acession
  • D86955
• Locus
  2q36.3
• Discovery year
  1997-02-10
• Entrez gene record
  6364
• Pubmed identfication
  • 9038201
  • 11352563
• RefSeq identity
  • NM_004591
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000133189

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