Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
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Catalog number
E-CL-H0816-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE3A , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBE3A . You can calculate the concentration of Human UBE3A in the samples by comparing the RLU value of the samples to the standard curve.
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Sensitivity
37.5pg/mL
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Detection Range
62.5~4000pg/mL
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Target Name
UBE3A
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UNIProt ID
NA
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
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Type
Sandwich
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbol
SNHG14, UBE3A
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Short name
UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
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Species
Human, Humans
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Alternative name
H. sapiens ubiquitin protein ligase E3A (Ubiquitin Protein Ligase E3A) CLIA reagent
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Alternative technique
kits
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Alternative to gene target
ubiquitin protein ligase E3A, ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, ligase activity, nuclei, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247,533136
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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