Human TERT (Telomerase Reverse Transcriptase) CLIA Kit
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Catalog number
E-CL-H0517-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT
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Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TERT . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TERT , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TERT . You can calculate the concentration of Human TERT in the samples by comparing the RLU value of the samples to the standard curve.
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Sensitivity
18.75pg/mL
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Detection Range
31.25~2000pg/mL
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Target Name
TERT
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UNIProt ID
NA
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human TERT (Telomerase Reverse Transcriptase) CLIA Kit
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Type
Sandwich
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Gene symbol
TERT
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Short name
TERT (Telomerase Reverse Transcriptase) CLIA Kit
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Technique
reverse
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Species
Human, Humans
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Alternative name
H. sapiens telomerase reverse transcriptase (Telomerase Reverse Transcriptase) CLIA reagent
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Alternative technique
transctription, kits
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Alternative to gene target
telomerase reverse transcriptase, CMM9 and DKCA2 and DKCB4 and EST2 and hEST2 and hTRT and PFBMFT1 and TCS1 and TP2 and TRT, TERT and IDBG-10998 and ENSG00000164362 and 7015, telomeric RNA binding, nuclei, Tert and IDBG-165912 and ENSMUSG00000021611 and 21752, BT.60929 and IDBG-632169 and ENSBTAG00000012567 and 518884
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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