Human SAA4(Serum Amyloid A4) EIA Kit
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Catalog number
E-EL-H5638-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human SAA4. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAA4. You can calculate the concentration of Human SAA4 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
46.88pg/mL
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Detection Range
78.13~5000pg/mL
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Target Name
SAA4
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UNIProt ID
P35542
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human SAA4(Serum Amyloid A4) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Sera
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About
Serum should be stored at +5°C
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Gene target
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Gene symbol
SAA2-SAA4, SAA4
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Short name
SAA4(Serum Amyloid A4) EIA Kit
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Technique
serum, EIA, eia
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Species
Human, Humans
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Alternative name
H. sapiens serum amyloid A4, constitutive(blood serum Amyloid A4) EIA reagent
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Alternative technique
sera, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
serum
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Gene info
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Identity
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Gene
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Long gene name
SAA2-SAA4 readthrough
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Locus
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Discovery year
2011-02-21
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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