Human MAdCAM1(Mucosal Addressin Cell Adhesion Molecule 1) EIA Kit
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Catalog number
E-EL-H1770-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human MAdCAM1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAdCAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAdCAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAdCAM1. You can calculate the concentration of Human MAdCAM1 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.1ng/mL
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Detection Range
0.16~10ng/mL
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Target Name
MAdCAM1
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UNIProt ID
Q13477
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human MAdCAM1(Mucosal Addressin Cell Adhesion Molecule 1) EIA Kit
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Type
Sandwich
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the MAdCAM1(Mucosal Addressin Adhesion Molecule 1) EIA Kit. For cells, cell lines and tissues in culture till half confluency. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Short name
MAdCAM1(Mucosal Addressin Adhesion Molecule 1) EIA Kit
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Technique
EIA, eia
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Species
Human, Humans
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Alternative name
H. sapiens mucosal vascular addressin cellular adhesion molecule 1(Mucosal Addressin cellular Adhesion Molecule 1) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
mucosal vascular addressin cell adhesion molecule 1, MACAM1, MADCAM1 and IDBG-12208 and ENSG00000099866 and 8174, protein binding, Plasma membranes, Madcam1 and IDBG-170375 and ENSMUSG00000020310 and 17123, BT.46202 and IDBG-645471 and ENSBTAG00000039355 and 527275,789849
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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