Human KRAS(V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog) EIA Kit
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Catalog number
E-EL-H2182-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human KRAS. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRAS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRAS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KRAS. You can calculate the concentration of Human KRAS in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.19ng/mL
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Detection Range
0.31~20ng/mL
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Target Name
KRAS
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UNIProt ID
L7RSL8
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human KRAS(V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
KRAS
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Short name
KRAS(V-Ki-Ras2 Kirsten Sarcoma Oncogene Homolog) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Humans, Rats
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Alternative name
H. sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog(V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Virus
sarcoma
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Process of growing viruses in live animals, plants, or cultured cells.
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Tree numbers
- E01.370.225.875.970
- E05.200.875.970
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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