Human IRF3(InterferonRegulatoryFactor3) EIAKit

• Catalog number
  E-EL-H2491-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human IRF3. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRF3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IRF3. You can calculate the concentration of Human IRF3 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  0.19ng/mL
• Detection Range
  0.31~20ng/mL
• Target Name
  IRF3
• UNIProt ID
  Q14653
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human IRF3(InterferonRegulatoryFactor3) EIAKit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  IRF3   InterferonRegulatoryFactor3   EIAKit  
• Gene symbol
  IRF3
• Short name
  IRF3(InterferonRegulatoryFactor3) EIAKit
• Species
  Human, Humans
• Alternative name
  H. sapiens interferon regulatory factor 3(InterferonRegulatoryFactor3) EIAKit
• Alternative to gene target
  interferon regulatory factor 3, IRF3 and IDBG-63225 and ENSG00000126456 and 3661, transcription regulatory region DNA binding, nuclei, Irf3 and IDBG-181123 and ENSMUSG00000003184 and 54131, IRF3 and IDBG-640634 and ENSBTAG00000006633 and 516979

Gene info

• Identity
  HGNC:6118
• Gene
  IRF3
• Long gene name
  interferon regulatory factor 3
• Locus
  19q13.33
• Discovery year
  1996-11-13
• Entrez gene record
  3661
• Pubmed identfication
  • 8524823
• RefSeq identity
  • NM_001571
• Classification
  • Interferon regulatory factors
• VEGA ID
  OTTHUMG00000183287

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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