Human GPX1(Glutathione Peroxidase 1) EIA Kit

• Catalog number
  E-EL-H5410-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Human
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human GPX1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPX1. You can calculate the concentration of Human GPX1 in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  18.75pg/mL
• Detection Range
  31.25~2000pg/mL
• Target Name
  GPX1
• UNIProt ID
  P07203
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Human GPX1(Glutathione Peroxidase 1) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugate
  Peroxidases with EC number 1.11.1.x are catalyzing the reaction of ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH

• Gene target
  GPX1   Glutathione   Kit  
• Gene symbol
  GPX1
• Short name
  GPX1(Glutathione 1) EIA Kit
• Technique
  EIA, eia
• Label
  peroxidase
• Species
  Human, Humans
• Alternative name
  H. sapiens glutathione peroxidase 1(GSH Peroxidase 1) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:4553
• Gene
  GPX1
• Long gene name
  glutathione peroxidase 1
• Synonyms name
  • selenoprotein GPX1
• Locus
  3p21.31
• Discovery year
  2001-06-22
• Entrez gene record
  2876
• Pubmed identfication
  • 9605859
• RefSeq identity
  • NM_000581
• Classification
  • Selenoproteins
• VEGA ID
  OTTHUMG00000156837

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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