Human GM-CSF-Ab(Anti-Granulocyte Macrophage Colony Stimulating Factor) EIA Kit

  • Catalog number
    E-EL-H1674-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Human
  • Target Synonym
    GM-CSF Ab
  • Test principle
    This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antigen specific to Human GM-CSF-Ab. Standards or samples are added to the micro EIA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Human GM-CSF-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM-CSF-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GM-CSF-Ab. You can calculate the concentration of Human GM-CSF-Ab in the samples by comparing the OD of the samples to the standard curve.
  • Sensitivity
    0.38ng/mL
  • Detection Range
    0.63~40ng/mL
  • Target Name
    GM-CSF-Ab
  • UNIProt ID
    NA
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Human GM-CSF-Ab(Anti-Granulocyte Macrophage Colony Stimulating Factor) EIA Kit
  • Type
    Sandwich
  • Technical data
  • Standard Curve RUL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Gene
    Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
  • Gene target
    GM-CSF-Ab   Granulocyte   Macrophage   Colony   Stimulating   Factor   Kit  
  • Gene symbol
    CSF2, PPP1R3A, CSF1, CSF3, IL3
  • Short name
    GM-CSF-Ab(Anti-Granulocyte Macrophage Colony Stimulating Factor) EIA Kit
  • Technique
    anti-, anti, EIA, antibody to, antibodies, eia
  • Species
    Human, Humans
  • Alternative name
    H. sapiens GM-CSF-Antibody(Antibody toGranulocyte Macrophage Colony Stimulating Factor) EIA reagent
  • Alternative technique
    antibodies, kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    granulocyte
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Blotting, Western
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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