Human GHRH(Growth Hormone Releasing Hormone) EIA Kit
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Catalog numberE-EL-H1146-96T
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Price:
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Size96Test
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Detection TypeColormetric
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Target SpeciesHuman
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Target SynonymGRF, GHRF, Somatoliberin, Somatocrinin
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human GHRH. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GHRH. You can calculate the concentration of Human GHRH in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity9.38pg/mL
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Detection Range15.63~1000pg/mL
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Target NameGHRH
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UNIProt IDP01286
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameHuman GHRH(Growth Hormone Releasing Hormone) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolGHRH
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Short nameGHRH(Growth Hormone Releasing Hormone) EIA Kit
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TechniqueEIA, eia
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SpeciesHuman, Humans
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Alternative nameH. sapiens GHRH(Growth Hormone Releasing Hormone) EIA reagent
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Alternative techniquekits, elisas
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namegrowth hormone releasing hormone
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Synonyms gene
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Neuropeptides
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data