Human F10(Coagulation Factor Ⅹ) EIA Kit
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Catalog numberE-EL-H0761-96T
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Price:
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Size96Test
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Detection TypeColormetric
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Target SpeciesHuman
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Target SynonymFactor X,eponym Stuart-Prower factor,prothrombinase,F10, FX, FXA
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human F10. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F10. You can calculate the concentration of Human F10 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity56.25pg/mL
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Detection Range93.75~6000pg/mL
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Target NameF10
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UNIProt IDP00742
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameHuman F10(Coagulation Factor Ⅹ) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbolF10, F10-AS1
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Short nameF10(Coagulation Factor Ⅹ) EIA Kit
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TechniqueEIA, eia
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SpeciesHumans
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Alternative nameH. sapiens F10(Coagulation Factor Ⅹ) EIA reagent
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Alternative techniquekits, elisas
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecoagulation factor X
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- Gla domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameF10 antisense RNA 1
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Synonyms gene name
- F10 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-05-09
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Classification
- Antisense RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data