Human AIRE(Autoimmune Regulator) EIA Kit
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Catalog number
E-EL-H0536-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
AIRE1, APECED, APS1, APSI, PGA1
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIRE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIRE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AIRE. You can calculate the concentration of Human AIRE in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.1ng/mL
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Detection Range
0.16~10ng/mL
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Target Name
AIRE
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UNIProt ID
O43918
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human AIRE(Autoimmune Regulator) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbol
AIRE
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Short name
AIRE(Autoimmune Regulator) EIA Kit
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Technique
EIA, eia
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Species
Human, Humans
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Alternative name
H. sapiens autoimmune regulator(Autoimmune Regulator) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
autoimmune regulator, AIRE1 and APECED and APS1 and APSI and PGA1, AIRE and IDBG-5152 and ENSG00000160224 and 326, translation regulator activity, nuclei, Aire and IDBG-168233 and ENSMUSG00000000731 and 11634, AIRE and IDBG-639674 and ENSBTAG00000023393 and
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Disease
autoimmune
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Gene info
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Identity
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Gene
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Long gene name
autoimmune regulator
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Synonyms gene
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Synonyms gene name
- autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1997-09-05
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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