HL-60 Cell Line
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Catalog numberEP-CL-0110
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationHL-60
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age36 years
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MorphologyMyeloblast
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Growth_propertiesSuspension
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Doubling_timeNA
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BackgroundHL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.
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Biosafety1
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MediumIMDM (PM150510)+20% FBS (164210-500)+1% P/S (PB180120)
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SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice(subcutaneous myeloid tumors). Yes, in semi-solid media.
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Receptor_expressionComplement, expressed; Fc, expressed
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Antigen_expressionNA
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Gene_expressionTumor Necrosis Factor(Tnf), Also Known As Tumor Necrosis Factor Alpha(Tnf-Alpha, Tnf Alpha), After Stimulation With Phorbol Myristic Acid, Myc+
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ApplicationsTransfection.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolHMGCL, IGLV4-60, IGHVII-60-1, IGHV3-60, RCC2, LIPC, ITLN2, OGFR, RNU6-60P, SPINK4
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Short nameHL-60 Line
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SpeciesHomo sapiens, Human
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Alternative nameHL-60 cellular Line
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TissuePeripheral blood
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Gene info
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Identity
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Gene
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Long gene name3-hydroxy-3-methylglutaryl-CoA lyase
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Synonyms gene name
- 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
- 3-hydroxymethyl-3-methylglutaryl-CoA lyase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-12-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 4-60
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-60-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-60-1
- immunoglobulin heavy variable (II)-60-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-60 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-60
- immunoglobulin heavy variable 3-60 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulator of chromosome condensation 2
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Synonyms
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Locus
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Discovery year2005-06-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelipase C, hepatic type
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Synonyms gene name
- lipase, hepatic
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- Lipases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintelectin 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-04-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibrinogen C domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameopioid growth factor receptor
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-31
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 60, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 60
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Locus
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Discovery year2011-08-08
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nameserine peptidase inhibitor Kazal type 4
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Synonyms gene name
- serine protease inhibitor, Kazal type 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-09-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serine peptidase inhibitors, Kazal type
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VEGA ID