HK-2 Cell Line

• Catalog number
  EP-CL-0109
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  HK-2
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  Adult
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317. Virus produced by the PA317 cells was used to transduce primary PTCs. Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. The cell line appears to be derived from a single cell based on Southern and FISH analysis. The E6/E7 genes are present in the HK-2 genome as determined by PCR. The cells retain a phenotype indicative of well differentiated PTCs. They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. The cells are negative for factor Ⅷ related antigen, 6. 19 antigen and CALLA endopeptidase. HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na＋dependent/phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. HK-2 cells are anchorage dependent. The cells will not grow in methylcellulose, soft agar or suspension. HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.
• Biosafety
  2
• Medium
  MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  Epidermal growth factor(EGF), expressed
• Antigen_expression
  NA
• Gene_expression
  Alkaline Phosphatase; Gamma Glutamyltranspeptidase; Leucine Aminopeptidase; Acid Phosphatase; Cytokeratin; Alpha 3, Beta 1 Integrin; Fibronectin
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  HK-2   Line  
• Gene symbol
  HK2, HOOK2, HK2-DT, KIF2A, KCNA5, TAC4, ATP12A, MIR7-2, MIR329-2, MIR512-2
• Short name
  HK-2 Line
• Species
  Homo sapiens, Human
• Alternative name
  HK-2 cellular Line
• Tissue
  Kidney, cortex/proximal tubule

Gene info

• Identity
  HGNC:4923
• Gene
  HK2
• Long gene name
  hexokinase 2
• Locus
  2p12
• Discovery year
  1993-03-11
• Entrez gene record
  3099
• RefSeq identity
  • NM_000189
• VEGA ID
  OTTHUMG00000129972

Gene info

• Identity
  HGNC:19885
• Gene
  HOOK2
• Long gene name
  hook microtubule tethering protein 2
• Synonyms gene name
  • hook homolog 2 (Drosophila)
• Synonyms
  • HK2
• GenBank acession
  • AF044924
• Locus
  19p13.13
• Discovery year
  2003-11-25
• Entrez gene record
  29911
• Pubmed identfication
  • 9927460
• RefSeq identity
  • NM_013312
• Classification
  • FHF complex
• VEGA ID
  OTTHUMG00000180372

Gene info

• Identity
  HGNC:55204
• Gene
  HK2-DT
• Long gene name
  HK2 divergent transcript
• Locus
  2p12
• Discovery year
  2020-08-21
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:6318
• Gene
  KIF2A
• Long gene name
  kinesin family member 2A
• Synonyms gene
  • KIF2
• Synonyms gene name
  • kinesin heavy chain member 2
• Synonyms
  • HK2
• GenBank acession
  • BC031828
• Locus
  5q12.1
• Discovery year
  1997-05-09
• Entrez gene record
  3796
• Pubmed identfication
  • 9177777
• RefSeq identity
  • NM_004520
• Classification
  • Kinesins
• VEGA ID
  OTTHUMG00000097755

Gene info

• Identity
  HGNC:6224
• Gene
  KCNA5
• Long gene name
  potassium voltage-gated channel subfamily A member 5
• Synonyms gene name
  • potassium voltage-gated channel, shaker-related subfamily, member 5
• Synonyms
  • Kv1.5
  • HK2
  • HPCN1
• GenBank acession
  • M83254
• Locus
  12p13.32
• Discovery year
  1991-08-13
• Entrez gene record
  3741
• Pubmed identfication
  • 16382104
• RefSeq identity
  • NM_002234
• Classification
  • Potassium voltage-gated channels
• VEGA ID
  OTTHUMG00000168232

Gene info

• Identity
  HGNC:16641
• Gene
  TAC4
• Long gene name
  tachykinin precursor 4
• Synonyms gene name
  • tachykinin 4
• Synonyms
  • HK-1
  • Pptc
  • PPT-C
• Synonyms name
  • hemokinin
• GenBank acession
  • AF521560
• Locus
  17q21.33
• Discovery year
  2004-10-20
• Entrez gene record
  255061
• Pubmed identfication
  • 11062498
  • 12383518
• RefSeq identity
  • NM_170685
• Classification
  • Tachykinin precursors
• VEGA ID
  OTTHUMG00000161830

Gene info

• Identity
  HGNC:13816
• Gene
  ATP12A
• Long gene name
  ATPase H+/K+ transporting non-gastric alpha2 subunit
• Synonyms gene
  • ATP1AL1
• Synonyms gene name
  • ATPase, Na+/K+ transporting, alpha polypeptide-like 1
  • ATPase, H+/K+ transporting, nongastric, alpha polypeptide
• Synonyms
  • HK
  • H-K-ATPase
• Synonyms name
  • ATPase, Na+K+ transporting, alpha-1 polypeptide-like
  • potassium-transporting ATPase alpha chain 2
  • proton pump
  • non-gastric H(+)/K(+) ATPase alpha subunit
  • sodium/potassium ATPase, alpha polypeptide-like
• GenBank acession
  • L42558
• Locus
  13q12.12
• Discovery year
  2000-11-09
• Entrez gene record
  479
• Pubmed identfication
  • 8838794
  • 2842249
  • 25322912
• RefSeq identity
  • NM_001676
• Classification
  • ATPase H+/K+ transporting
• VEGA ID
  OTTHUMG00000016588

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

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