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Abbreviation
Hela
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
31 years adult
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
The cells are positive for keratin by immunoperoxidase staining.HeLa cells have been reported to contain human papilloma virus 18(HPV-18) sequences.P53 expression was reported to be low, and normal levels of pRB(retinoblastoma suppressor) were found.
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Biosafety
2
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Medium
MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
Lysophosphatidylcholine(Lyso-Pc) Induces Ap-1 Activity And C-Jun N-Terminal Kinase Activity(Jnk1) By A Protein Kinase C-Independent Pathway. The Cells Are Positive For Keratin By Immunoperoxidase Staining.
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.