HEC-1-A Cell Line
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Catalog numberEP-CL-0099
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationHEC-1-A
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age71 years
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundPAF induces increased expression of c-fos.
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Biosafety1
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MediumMcCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice. The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma(gradeⅡ). Yes, in the cheek pouch of cortisone treated hamsters. The cells form typical papillary adenomas.
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Receptor_expressionReceptor expression: platelet activating factor(PAF)
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Antigen_expressionBlood Type B; Rh＋
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Gene_expressionOncogenes: C-Fos＋
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolIGHV5-10-1, NR1H3, SPI1, H2AC8, HLA-A, CHST4, NDC80, F11R, CIDEA, RPA1
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Short nameHEC-1-A Line
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SpeciesHomo sapiens, Human
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Alternative nameHEC-1-A cellular Line
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TissueUterus, endometrium
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear receptor subfamily 1 group H member 3
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Synonyms gene name
- nuclear receptor subfamily 1, group H, member 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-17
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Entrez gene record
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Pubmed identfication
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Classification
- Nuclear receptor subfamily 1 group H
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSpi-1 proto-oncogene
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Synonyms gene name
- spleen focus forming virus (SFFV) proviral integration oncogene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-07-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2A clustered histone 8
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Synonyms gene
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Synonyms gene name
- H2A histone family, member A
- histone 1, H2ae
- histone cluster 1, H2ae
- histone cluster 1 H2A family member e
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2A histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecarbohydrate sulfotransferase 4
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Synonyms gene name
- carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sulfotransferases, membrane bound
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNDC80 kinetochore complex component
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Synonyms gene
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Synonyms gene name
- highly expressed in cancer, rich in leucine heptad repeats (yeast)
- kinetochore associated 2
- NDC80 kinetochore complex component homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- NDC80 kinetochore complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameF11 receptor
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Synonyms gene
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Synonyms gene name
- junctional adhesion molecule 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- V-set domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
