-
Abbreviation
HCT 116
-
Leadtime
3-5 days
-
Shipping condition
Dry ice
-
Storage
Liquid nitrogen -196°C
-
Age
Adult
-
Morphology
Epithelial
-
Growth_properties
Adherent
-
Doubling_time
NA
-
Background
The cells are positive for keratin by immunoperoxidase staining.HCT 116 cells are positive for transforming growth factor beta 1(TGF beta 1) and beta 2(TGF beta 2) expression.
-
Biosafety
1
-
Medium
McCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
-
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio
1:2-1:4
-
Renewal
Every 2 to 3 days
-
Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
Tumorigenic
Yes
-
Effects
Yes, in nude mice.
-
Receptor_expression
NA
-
Antigen_expression
NA
-
Gene_expression
Carcinoembryonic Antigen(Cea) 1Ng Per 10^6 Cells Per 10 Days.
-
Applications
This cell line is a suitable transfection host. This line has a mutation in codon 13 of the ras proto-oncogene, and can be used as a positive control for PCR assays of mutation in this codon.
-
Duration
NA
-
-
Description
For cells, cell lines and tissues in culture till half confluency.
