HBL-100 Cell Line

• Catalog number
  EP-CL-0091
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  HBL-100
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  27 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The epithelial cell line HBL-100 has been derived by E.V. Gaffney and associates fromthe milk of a nursing mother and obtained 3 days after delivery. Although there was noevidence of a breast lesion in the milk donor, and the patient had no family history ofbreast cancer, the karyotype of the recovered cells was abnormal as early as passage 7.This line was able to synthesize a small amount of lactose and would respond toprolactin or estrogen by producing increased amounts of casein. Electron micrographsrevealed microvilli, tonofibrils and desmosomes.
• Biosafety
  2
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in nude mice. At passage levels below 35 the line is not tumorigenic in nude mice, but forms colonies in soft agar. Tumorigenicity has been reported to increase above passage 35.
• Receptor_expression
  NA
• Antigen_expression
  HLA A1, A10, A11, B7, B8
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  HBL-100   Line  
• Gene symbol
  SCARNA3, TMED5, MIR100, PVT1, EXOSC10
• Short name
  HBL-100 Line
• Species
  Homo sapiens, Human
• Alternative name
  HBL-100 cellular Line
• Tissue
  Breast

Gene info

• Identity
  HGNC:32577
• Gene
  SCARNA3
• Long gene name
  small Cajal body-specific RNA 3
• Synonyms
  • HBI-100
• Locus
  1q25.1
• Discovery year
  2006-03-31
• Entrez gene record
  677679
• Pubmed identfication
  • 11387227
• RefSeq identity
  • NR_002998
• Classification
  • Small Cajal body-specific RNAs

Gene info

• Identity
  HGNC:24251
• Gene
  TMED5
• Long gene name
  transmembrane p24 trafficking protein 5
• Synonyms gene name
  • transmembrane emp24 protein transport domain containing 5
• Synonyms
  • CGI-100
  • p24g2
  • p24gamma2
• GenBank acession
  • BC070051
• Locus
  1p22.1
• Discovery year
  2004-12-21
• Entrez gene record
  50999
• Pubmed identfication
  • 10810093
• RefSeq identity
  • NM_016040
• Classification
  • Transmembrane p24 trafficking proteins
• VEGA ID
  OTTHUMG00000010162

Gene info

• Identity
  HGNC:31487
• Gene
  MIR100
• Long gene name
  microRNA 100
• Synonyms gene
  • MIRN100
• Synonyms
  • hsa-mir-100
• Locus
  11q24.1
• Discovery year
  2004-04-23
• Entrez gene record
  406892
• RefSeq identity
  • NR_029515
• Classification
  • MicroRNA MIR10/100 family

Gene info

• Identity
  HGNC:9709
• Gene
  PVT1
• Long gene name
  Pvt1 oncogene
• Synonyms gene name
  • pvt-1 (murine) oncogene homolog, MYC activator
  • Pvt1 oncogene homolog (mouse)
  • Pvt1 oncogene (non-protein coding)
• Synonyms
  • NCRNA00079
  • LINC00079
  • onco-lncRNA-100
  • MIR1204HG
• Synonyms name
  • non-protein coding RNA 79
  • long intergenic non-protein coding RNA 79
  • MIR1204, MIR1205, MIR1206 and MIR1207 host gene
  • plasmacytoma variant translocation 1
• GenBank acession
  • M31519
• Locus
  8q24.21
• Discovery year
  2001-06-22
• Entrez gene record
  5820
• Pubmed identfication
  • 17503467
  • 23801869
• RefSeq identity
  • NR_003367
• Classification
  • Long non-coding RNAs with non-systematic symbols
  • MicroRNA non-coding host genes
• VEGA ID
  OTTHUMG00000164871

Gene info

• Identity
  HGNC:9138
• Gene
  EXOSC10
• Long gene name
  exosome component 10
• Synonyms gene
  • PMSCL2
• Synonyms gene name
  • polymyositis/scleroderma autoantigen 2, 100kDa
• Synonyms
  • PM-Scl
  • PM/Scl-100
  • Rrp6p
  • RRP6
  • p2
  • p3
  • p4
• Synonyms name
  • polymyositis/scleroderma autoantigen 2 (100kD)
• GenBank acession
  • BC073788
• Locus
  1p36.22
• Discovery year
  1994-09-19
• Entrez gene record
  5394
• Pubmed identfication
  • 1383382
  • 1644924
• RefSeq identity
  • NM_001001998
• Classification
  • Exosome complex
• VEGA ID
  OTTHUMG00000002123

Similar products