H-4-II-E [H4-II-E] Cell Line

Abbreviation

H-4-II-E [H4-II-E]
Leadtime

3-5 days
Shipping condition

Dry ice
Storage

Liquid nitrogen -196°C
Age

NA
Morphology

Epithelial
Growth_properties

Adherent
Doubling_time

NA
Background

The line has been cured of Mycoplasma arginini contamination.
Biosafety

1
Medium

MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio

1:2-1:4
Renewal

Every 2 to 3 days
Cryopreservation

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture_conditions

Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic

NA
Effects

NA
Receptor_expression

NA
Antigen_expression

NA
Gene_expression

NA
Applications

The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Duration

NA
Technical data
Description

For cells, cell lines and tissues in culture till half confluency.

Identity

HGNC:4785
Gene

H4C11
Long gene name

H4 clustered histone 11
Synonyms gene
- H4FE
- HIST1H4J
Synonyms gene name
- H4 histone family, member E
- histone 1, H4j
- histone cluster 1, H4j
- histone cluster 1 H4 family member j
Synonyms
- H4/e
- H4F2iv
GenBank acession
- J00188
Locus

6p22.1
Discovery year

1998-09-07
Entrez gene record

8363
Pubmed identfication
- 6265100
- 9439656
- 12408966
RefSeq identity
- NM_021968
Classification
- H4 histones
VEGA ID

OTTHUMG00000014487

Identity

HGNC:14011
Gene

SMC2
Long gene name

structural maintenance of chromosomes 2
Synonyms gene
- SMC2L1
Synonyms gene name
- SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
- SMC2 structural maintenance of chromosomes 2-like 1 (yeast)
Synonyms
- hCAP-E
- CAP-E
GenBank acession
- AF092563
Locus

9q31.1
Discovery year

2001-02-22
Entrez gene record

10592
Pubmed identfication
- 9789013
Classification
- Condensin I subunits
- Condensin II subunits
- Structural maintenance of chromosomes proteins
VEGA ID

OTTHUMG00000020401

Identity

HGNC:6376
Gene

KLRC3
Long gene name

killer cell lectin like receptor C3
Synonyms gene name
- killer cell lectin-like receptor subfamily C, member 3
Synonyms
- NKG2-E
GenBank acession
- L14542
Locus

12p13.2
Discovery year

1998-01-16
Entrez gene record

3823
Pubmed identfication
- 9598306
RefSeq identity
- NM_002261
Classification
- C-type lectin domain containing
- Killer cell lectin like receptors
VEGA ID

OTTHUMG00000167149

Identity

HGNC:11840
Gene

TLE4
Long gene name

TLE family member 4, transcriptional corepressor
Synonyms gene name
- transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
- transducin like enhancer of split 4
Synonyms
- E(spl)
- ESG
- GRG4
GenBank acession
- M99439
Locus

9q21.31
Discovery year

1993-12-20
Entrez gene record

7091
Pubmed identfication
- 8365415
RefSeq identity
- NM_001282748
Classification
- WD repeat domain containing
- TLE family
- Wnt enhanceosome complex
VEGA ID

OTTHUMG00000020072