GBC-SD Cell Line

• Catalog number
  EP-CL-0085
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  GBC-SD
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  NA
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, forms tumors in nude mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  GBC-SD   Line  
• Gene symbol
  RANGAP1, SLC17A5
• Short name
  GBC-SD Line
• Species
  Homo sapiens, Human
• Alternative name
  GBC-SD cellular Line
• Tissue
  Gallbladder

Gene info

• Identity
  HGNC:9854
• Gene
  RANGAP1
• Long gene name
  Ran GTPase activating protein 1
• Synonyms gene
  • SD
• Synonyms gene name
  • segregation distorter homolog (Drosophila)
• Synonyms
  • Fug1
  • KIAA1835
• GenBank acession
  • X82260
• Locus
  22q13.2
• Discovery year
  1998-02-26
• Entrez gene record
  5905
• Pubmed identfication
  • 7878053
• RefSeq identity
  • NM_002883
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000150940

Gene info

• Identity
  HGNC:10933
• Gene
  SLC17A5
• Long gene name
  solute carrier family 17 member 5
• Synonyms gene
  • SIASD
• Synonyms gene name
  • sialic acid storage disease
  • solute carrier family 17 (anion/sugar transporter), member 5
  • solute carrier family 17 (acidic sugar transporter), member 5
• Synonyms
  • AST
  • SD
  • ISSD
  • NSD
  • SIALIN
  • SLD
• GenBank acession
  • AJ387747
• Locus
  6q13
• Discovery year
  2000-01-06
• Entrez gene record
  26503
• Pubmed identfication
  • 10581036
  • 8198127
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000015039

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