FO Cell Line
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Catalog numberEP-CL-0337
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationFO
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyLymphoblast
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Growth_propertiesSuspension
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Doubling_timeNA
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BackgroundThe cells are resistant to 8-azaguanine and do not produce immunoglobulin.Tested and found negative for ectromelia virus(mousepox).
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Biosafety1
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MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsThe line can be used as a fusion partner for making B cell hybridomas.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short nameFO Line
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SpeciesMus musculus, Mouse
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Alternative nameFO cellular Line
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TissueMyeloma
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