EL4 Cell Line

• Catalog number
  EP-CL-0078
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  EL4
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Lymphoblast
• Growth_properties
  Suspension
• Doubling_time
  NA
• Background
  The cells are resistant to 0. 1mM cortisol and sensitive to 20 μg/mL PHA. A subline(EL4. IL-2) that produces high levels of interleukin-2(IL-2, interleukin 2) is available. A subline(EL4. IL-2) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine(BUdR) is available. A subline(EL4. BU. 1. OUAr. 1. 1) that is resistant to 0. 1mM 5-bromo-2'-deoxyuridine and 1mM ouabain is available. Tested and found negative for ectromelia virus(mousepox).
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% HS (164215-100)+1% P/S (PB180120)
• Subculturing
  Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  H-2b; Thy-1.2
• Gene_expression
  NA
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  EL4   Line  
• Short name
  EL4 Line
• Species
  Mus musculus, Mouse
• Alternative name
  EL4 cellular Line
• Tissue
  Peripheral blood

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