cGMP(Cyclic GMP) EIA Kit

Detection Type

Colormetric
Target Species

Universal
Target Synonym

NA
Test principle

This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Sensitivity

0.47pmol/mL
Detection Range

0.78~50pmol/mL
Target Name

cGMP
UNIProt ID

NA
Tested Sample Types

Serum, Plasma, Cell supernatant
Product Name

cGMP(Cyclic GMP) EIA Kit
Type

Competitive
Technical data
Standard Curve RUL
Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

Gene target

cGMP Cyclic GMP Kit
Gene symbol

NKG7, NT5C2
Short name

cGMP(Cyclic GMP) EIA Kit
Technique

EIA, eia
Alternative name

cGMP(Cyclic GMP) EIA reagent
Alternative technique

kits, elisas
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:8022
Gene

NT5C2
Long gene name

5'-nucleotidase, cytosolic II
Synonyms gene
- NT5B
- SPG45
Synonyms gene name
- 5'-nucleotidase (purine), cytosolic type B
- spastic paraplegia 45 (autosomal recessive)
Synonyms
- PNT5
- GMP
- cN-II
- SPG65
Synonyms name
- purine 5' nucleotidase
GenBank acession
- D38524
Locus

10q24.32-q24.33
Discovery year

2000-06-16
Entrez gene record

22978
Pubmed identfication
RefSeq identity
- NM_012229
Classification
- 5'-nucleotidases
VEGA ID

OTTHUMG00000018981

Name

Immunoenzyme Techniques
Concept

Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data