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Abbreviation
Caco-2
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
72 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
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Biosafety
1
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Medium
MEM (PM150410)+20% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
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Receptor_expression
This cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
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Antigen_expression
NA
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Gene_expression
Keratin, Retinoic Acid Binding Protein 1, Retinol Binding Protein 2
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Applications
Transfection host.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
