AsPC-1 Cell Line

• Catalog number
  EP-CL-0027
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  AsPC-1
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  62 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:3-1:6
• Renewal
  2 to 3 times per week
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Carcinoembryonic Antigen(Cea); Human Pancreas Associated Antigen; Human Pancreas Specific Antigen; Mucin
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  AsPC-1   Line  
• Short name
  AsPC-1 Line
• Species
  Homo sapiens, Human
• Alternative name
  AsPC-1 cellular Line
• Tissue
  Pancreas, derived from metastatic site: ascites

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