Affinity purified Rat IgG2a Isotype Antibody FITC-Conjugated

• Catalog number
  ERIS008-200
• Price
  Please ask
• Size
  200 mg

• Clone
  2A3
• Source
  Rat
• Clonality
  Monoclonal
• Category
  Isotype Control
• Description
  Rat IgG2a was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
• Reactivity
  Not specified / Inquire
• Conjugation
  FITC
• Purification
  Affinity purified
• Antibody s supplied with
  PBS pH 7.2, 0.09% NaN3, 0.2% BSA
• Antibody s concentration
  0,5 mg/ml
• Storage
  Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
• Antibody s is used for
  Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
• Specifications
• Test
  A high affinity purification column was use to purify IgG2a Isotype Antibody FITC-Conjugated by Elabscience by chromatographic size exclusion.
• Additional description
  1 of protein A or G purified by Elabscience will give more specificity than crude serum.
• Properties
  If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Additional conjugation
  Anti-FITC Antibody
• Group
  isotypes
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• French translation
  anticorps

• Gene target
  IgG2a   Conjugated  
• Short name
  IgG2a Isotype Antibody FITC-Conjugated
• Technique
  affinity, Antibody, purified, Isotype, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Isotypes and controls, purifications
• Host
  Rat
• Isotype
  IgG2a, IgG2a
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  protein enriched Rat IgG2a isoform (antibody to-) fluorecein-coupled
• Alternative technique
  pure, antibodies, control, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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