769-P Cell Line

• Catalog number
  EP-CL-0009
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  769-P
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  63 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  ~35 hours
• Background
  The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.They can be grown in soft agar.
• Biosafety
  1
• Medium
  RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:4-1:8
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, forms colonies in soft agar; Yes, forms tumors in immunosuppressed hamsters; Yes, forms tumors in nude mice.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  769-P   Line  
• Gene symbol
  RNU6-769P, MIR769, HMGA1P8, PREX1, RASSF9, ABCB1, DLG5, HLA-P, SMR3B, CENPP
• Short name
  769-P Line
• Species
  Homo sapiens, Human
• Alternative name
  769-P cellular Line
• Tissue
  Kidney

Gene info

• Identity
  HGNC:47732
• Gene
  RNU6-769P
• Long gene name
  RNA, U6 small nuclear 769, pseudogene
• Locus
  12q13.12
• Discovery year
  2013-05-01
• Entrez gene record
  106479906

Gene info

• Identity
  HGNC:33138
• Gene
  MIR769
• Long gene name
  microRNA 769
• Synonyms gene
  • MIRN769
• Synonyms
  • hsa-mir-769
• Locus
  19q13.32
• Discovery year
  2006-10-10
• Entrez gene record
  768217
• Pubmed identfication
  • 25081069
  • 27470346
• RefSeq identity
  • NR_030412
• Classification
  • MicroRNAs

Gene info

• Identity
  HGNC:39183
• Gene
  HMGA1P8
• Long gene name
  high mobility group AT-hook 1 pseudogene 8
• Synonyms
  • HMGA1-p
• Locus
  2p13.1
• Discovery year
  2010-11-17
• Entrez gene record
  100130009
• Pubmed identfication
  • 12727900
  • 20975707
• RefSeq identity
  • NG_032791
• VEGA ID
  OTTHUMG00000152827

Gene info

• Identity
  HGNC:32594
• Gene
  PREX1
• Long gene name
  phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1
• Synonyms gene name
  • phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
• Synonyms
  • KIAA1415
  • P-REX1
• Synonyms name
  • PIP3 dependent Rac exchange factor 1
• GenBank acession
  • AB037836
• Locus
  20q13.13
• Discovery year
  2008-09-15
• Entrez gene record
  57580
• Pubmed identfication
  • 11955434
  • 15545267
  • 16301320
• RefSeq identity
  • NM_020820
• Classification
  • PDZ domain containing
  • Dbl family Rho GEFs
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000032685

Gene info

• Identity
  HGNC:15739
• Gene
  RASSF9
• Long gene name
  Ras association domain family member 9
• Synonyms gene
  • PAMCI
• Synonyms gene name
  • peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor
  • Ras association (RalGDS/AF-6) domain family (N-terminal) member 9
• Synonyms
  • P-CIP1
• Locus
  12q21.31
• Discovery year
  2001-05-23
• Entrez gene record
  9182
• Pubmed identfication
  • 9837933
  • 18272789
• Classification
  • Ras association domain family
• VEGA ID
  OTTHUMG00000169831

Gene info

• Identity
  HGNC:40
• Gene
  ABCB1
• Long gene name
  ATP binding cassette subfamily B member 1
• Synonyms gene
  • PGY1
  • MDR1
  • CLCS
• Synonyms gene name
  • colchicin sensitivity
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Synonyms
  • P-gp
  • CD243
  • GP170
  • ABC20
  • p-170
• Synonyms name
  • multidrug resistance protein 1
• GenBank acession
  • M14758
• Locus
  7q21.12
• Discovery year
  1986-01-01
• Entrez gene record
  5243
• Pubmed identfication
  • 3027054
• RefSeq identity
  • NM_000927
• Classification
  • ATP binding cassette subfamily B
  • CD molecules
• VEGA ID
  OTTHUMG00000023393

Gene info

• Identity
  HGNC:2904
• Gene
  DLG5
• Long gene name
  discs large MAGUK scaffold protein 5
• Synonyms gene name
  • discs, large homolog 5 (Drosophila)
  • discs large homolog 5
• Synonyms
  • P-dlg
  • KIAA0583
• GenBank acession
  • U61843
• Locus
  10q22.3
• Discovery year
  1999-02-23
• Entrez gene record
  9231
• Pubmed identfication
  • 9738934
• RefSeq identity
  • NM_004747
• Classification
  • Membrane associated guanylate kinases
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000018548

Gene info

• Identity
  HGNC:21196
• Gene
  HLA-P
• Long gene name
  major histocompatibility complex, class I, P (pseudogene)
• Synonyms gene
  • C6orf101
  • HLA-90
• Synonyms gene name
  • chromosome 6 open reading frame 101
  • HLA-90 pseudogene
• Synonyms
  • dJ377H14.3
• GenBank acession
  • M96333
• Locus
  6p22.1
• Discovery year
  2003-11-26
• Entrez gene record
  352963
• RefSeq identity
  • NG_002731
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000172781
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:17326
• Gene
  SMR3B
• Long gene name
  submaxillary gland androgen regulated protein 3B
• Synonyms gene
  • PROL3
• Synonyms gene name
  • proline rich 3
  • submaxillary gland androgen regulated protein 3 homolog B (mouse)
• Synonyms
  • P-B
  • PRL3
• GenBank acession
  • D29833
• Locus
  4q13.3
• Discovery year
  2002-01-03
• Entrez gene record
  10879
• Pubmed identfication
  • 7982889
  • 479131
• RefSeq identity
  • NM_006685
• VEGA ID
  OTTHUMG00000129396

Gene info

• Identity
  HGNC:32933
• Gene
  CENPP
• Long gene name
  centromere protein P
• Synonyms
  • RP11-19J3.3
  • CENP-P
• GenBank acession
  • AK091247
• Locus
  9q22.31
• Discovery year
  2006-06-15
• Entrez gene record
  401541
• Pubmed identfication
  • 16622419
  • 16622420
• RefSeq identity
  • NM_001012267
• Classification
  • Constitutive centromere associated network
• VEGA ID
  OTTHUMG00000020228

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