Rabbit Anti Zebrafish ascl1a Antibody-Biotin

• Catalog number
  CSB-PA852654LD01DIL_50ug
• Price
  Please ask
• Size
  50ug

• Long name
  Rabbit Antibody against Zebrafish ascl1a, Biotin
• Synonym name
  ascl1a Zebrafish Antibody
• Species reactivity
  Zebrafish
• Tested applications recommended dilutions
  ELISA
• Conjugation
  Biotin
• Antibody isotype
  IgG
• UniProt ID
  Q90259
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant Zebrafish Achaete-scute homolog 1a protein (1-196AA)
• Immunogen species
  Zebrafish
• Alias
  Achaete-scute homolog 1a,Zash-1a,Pituitary-absent protein,ascl1a
• Background
  Transcriptional regulator. May mediate transcription activation by binding to the E box-containing promoter (By similarity). Involved in neurogenesis. Required for the development of neurons in the epiphysis, acting partially redundantly with neurog1 and downstream of flh. Involved in maintaining rhombomere boundaries in the hindbrain, probably via up-regulation of delta expression. Also involved in pituitary development; required cell-autonomously in adenohypophyseal cells for endocrine differentiation and for survival of a subset of cells.
• Clonality
  Polyclonal antibody
• Shipping and storage
  ascl1a Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Short name of the antigen
  Achaete-scute homolog 1a
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  Zebrafish   ascl1a  
• Short name
  Anti-ascl1a
• Technique
  Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit, Rabbits
• Label
  Biotin
• Species
  Zebrafish, Zebrafishes
• Alternative name
  ascl1a Antibody,Biotin conjugated
• Alternative technique
  antibodies, rabbit-anti

MeSH Data

• Name
  In Situ Hybridization
• Concept
  Scope note: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
• Tree numbers
  • E01.370.225.500.620.670.325
  • E01.370.225.750.600.670.325
  • E05.200.500.620.670.325
  • E05.200.750.600.670.325
  • E05.393.661.475
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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