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Long name
Rabbit Antibody against Rat Nos3, FITC
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Synonym name
Nos3 Rat Antibody
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Species reactivity
Rat
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Tested applications recommended dilutions
ELISA
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Conjugation
FITC
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Antibody isotype
IgG
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UniProt ID
Q62600
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant rat Nitric oxide synthase, endothelial protein (519-690AA)
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Immunogen species
Rat
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Alias
Constitutive NOS,EC-NOS,Endothelial NOS,Nos3
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Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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Clonality
Polyclonal antibody
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Shipping and storage
Nos3 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Nitric oxide synthase, endothelial protein
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
NANOS3, NOS3
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Short name
Anti-Nos3
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Technique
Antibody, Rabbit, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Label
FITC
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Species
Rat, Rats
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Alternative name
Nos3 Antibody,FITC conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Alternative to gene target
nitric oxide synthase 3 (endothelial cell), ECNOS and eNOS, NOS3 and IDBG-48571 and ENSG00000164867 and 4846, NADP binding, nuclei, Nos3 and IDBG-140461 and ENSMUSG00000028978 and 18127, NOS3 and IDBG-637485 and ENSBTAG00000017680 and 287024
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