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Long name
Rabbit Antibody against Mouse Angpt2, Biotin
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Synonym name
Angpt2 Mouse Antibody
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Species reactivity
Mouse
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
O35608
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant mouse Angiopoietin-2 protein (19-483AA)
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Immunogen species
Mouse
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Alias
Angpt2,Agpt2
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Background
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal
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Clonality
Polyclonal antibody
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Shipping and storage
Angpt2 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Angiopoietin-2 protein
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translation
anticorps
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Gene target
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Gene symbol
ANGPT2
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Short name
Anti-Angpt2
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Technique
anti mouse, Antibody, Rabbit, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
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Host
Rabbit, Rabbits
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
Angpt2 Antibody,Biotin conjugated
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Alternative technique
antibodies, rabbit-anti, murine
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Alternative to gene target
angiopoietin 2, AGPT2 and ANG2, ANGPT2 and IDBG-5893 and ENSG00000091879 and 285, metal ion binding, nuclei, Angpt2 and IDBG-137356 and ENSMUSG00000031465 and 11601, ANGPT2 and IDBG-628426 and ENSBTAG00000011034 and 282141
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