-
Long name
Rabbit Antibody against Human, Rat HMGCL, Non-conjugated
-
Synonym name
HMGCL Human, Rat Antibody
-
Species reactivity
Human,Rat
-
Tested applications recommended dilutions
ELISA,WB,IHC;Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200
-
Conjugation
Non-conjugated
-
Antibody isotype
IgG
-
UniProt ID
P35914
-
Format
Liquid, diluted in buffer
-
Buffer composition
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Purity
>95%
-
Purification method
Antigen Affinity Purified Antibodies
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant human Hydroxymethylglutaryl-CoA lyase, mitochondrial protein (1-325AA)
-
Immunogen species
Human
-
Alias
Hydroxymethylglutaryl-CoA lyase,mitochondrial,HL,HMG-CoA lyase,3-hydroxy-3-methylglutarate-CoA lyase,HMGCL
-
Background
Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
-
Clonality
Polyclonal antibody
-
Shipping and storage
HMGCL Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Short name of the antigen
Hydroxymethylglutaryl-CoA lyase, mitochondrial
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin name
Oryctolagus cuniculus, Rattus norvegicus
-
French translation
anticorps
