-
Long name
Rabbit Antibody against Human, Mouse, Rat ATP5B, Non-conjugated
-
Synonym name
ATP5B Human, Mouse, Rat Antibody
-
Species reactivity
Human,Mouse,Rat
-
Tested applications recommended dilutions
ELISA,WB,IHC;Recommended dilution:WB:1:500-1:5000,IHC:1:20-1:200
-
Conjugation
Non-conjugated
-
Antibody isotype
IgG
-
UniProt ID
P06576
-
Format
Liquid, diluted in buffer
-
Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
-
Purity
>95%
-
Purification method
Protein G purified
-
Antibody category
Polyclonal Antibodies
-
Immunogen type
Recombinant Human ATP synthase subunit beta, mitochondrial protein (230-529AA)
-
Immunogen species
Human
-
Alias
ATP synthase subunit beta,mitochondrial,ATP5B,ATPMB,ATPSB
-
Background
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
-
Clonality
Polyclonal antibody
-
Shipping and storage
ATP5B Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
-
Short name of the antigen
ATP synthase subunit beta, mitochondrial
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
-
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
French translation
anticorps
-
Gene target
-
Gene symbol
ATP5F1B
-
Short name
Anti-ATP5B
-
Technique
anti human, Antibody, Rabbit, Mouse, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
-
Host
Rabbit, Rabbits
-
Species
Rat, Humans, Mouses, Rats
-
Alternative name
ATP5B Antibody
-
Alternative technique
antibodies, rabbit-anti, murine
-
Alternative to gene target
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB and ATPSB and HEL-S-271, ATP5B and IDBG-41395 and ENSG00000110955 and 506, hydrolase activity, nuclei, Atp5b and IDBG-196497 and ENSMUSG00000025393 and 11947, ATP5B and IDBG-635890 and ENSBTAG00000013315 and 327675
-
