Rabbit Anti Human,Mouse RAB5A Antibody-Non-conjugated
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Catalog numberCSB-PA019213ESR1HU_100ul
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Price:
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Size100ul
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Long nameRabbit Antibody against Human, Mouse RAB5A, Non-conjugated
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Synonym nameRAB5A Human, Mouse Antibody
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Species reactivityHuman,Mouse
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Tested applications recommended dilutionsELISA,WB,IHC;Recommended dilution:WB:1:500-5000,IHC:1:20-1:200
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ConjugationNon-conjugated
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Antibody isotypeIgG
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UniProt IDP20339
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FormatLiquid, diluted in buffer
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Buffer compositionPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Purity>95%
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Purification methodAntigen Affinity Purified Antibodies
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant human Ras-related protein Rab-5A protein (1-215AA)
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Immunogen speciesHuman
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AliasRAB5A,RAB5
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BackgroundThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension.
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ClonalityPolyclonal antibody
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Shipping and storageRAB5A Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Oryctolagus cuniculus
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translationanticorps
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Gene target
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Gene symbolRAB5A
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Short nameAnti-RAB5A
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Techniqueanti human, Antibody, Rabbit, Mouse, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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HostRabbit, Rabbits
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SpeciesMouse, Humans, Mouses
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Alternative nameRAB5A Antibody
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Alternative techniqueantibodies, rabbit-anti, murine
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Alternative to gene targetRAB5A, member RAS oncogene family, RAB5, RAB5A and IDBG-21702 and ENSG00000144566 and 5868, GDP-dissociation inhibitor binding, nuclei, Rab5a and IDBG-190768 and ENSMUSG00000017831 and 271457, BT.24043 and IDBG-631304 and ENSBTAG00000046385 and 539764
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Gene info
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Identity
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Gene
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Long gene nameRAB5A, member RAS oncogene family
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Synonyms gene
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Synonyms name
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Locus
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Discovery year1990-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data